Delineating cell lineage in the collecting duct of mouse kidney

The collecting duct in the kidney consists in three types of cells, principal cells, alpha-intercalated cells and beta-intercalated cells. The principal cells are primarily responsible for salt and water transport while alpha- and beta-intercalated cells are responsible for acid-base transport.

Figure legend

Anti-Aqp2 antibody labels the luminal membrane of the principal cells in the collecting duct while anti-Pendrin antibody labels the luminal membrane of the beta-intercalated cells in the collecting duct. Note that Pendrin is found in the distal tubules beside the collecting ducts where there are no Aqp2 labeling.

In the collecting duct, there are two types of intercalated cells: alpha- and beta-intercalated cells. Beta-intercalated cells can be labeled with anti-Pendrin antibody while alpha-intercalated cells can be labeled with anti-ATP6B1 antibody.

Figure legend

Anti-Pendrin antibody labels the luminal membrane of the beta-intercalated cells while anti-ATP6B1 antibody labels the luminal membrane of the alpha-intercalated cells. Note that anti-ATP6B1 antibody also labels the luminal and the basolateral membrane of the beta-intercalated cells.