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Protocol for Staining WNK Protein

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  • WNK1 antibody labeling of mouse tissue

    WNK1 antibody

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    Antibody: Rabbit WNK1 Polyclonal Antibody

    Concentration: 0.25 mg/ml purified IgG

    Application: Validated by immunofluorescence labeling (1:100)

    Reactivity: Human, mouse, rat

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  • WNK2 antibody labeling of mouse hippocampus

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    Antibody: Rat WNK2 Polyclonal Antibody

    Concentration: 0.25 mg/ml purified IgG

    Application: Validated by immunofluorescence labeling (1:100)

    Reactivity: Human, mouse, rat

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  • WNK3 antibody of mouse brain glia

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    Item Cat No.: 50003

    Antibody: Rat WNK3 Polyclonal Antibody

    Concentration: 0.25 mg/ml purified IgG

    Application: Validated by immunofluorescence labeling (1:100)

    Reactivity: Human, mouse, rat

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  • WNK4 antibody labeling of mouse kidney

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    WNK4 antibody

    Item Cat No.: 50004

    Antibody: Rabbit WNK4 Polyclonal Antibody

    Concentration: 0.25 mg/ml purified IgG

    Application: Validated by immunofluorescence labeling (1:100)

    Reactivity: Human, mouse, rat

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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