Cryosections are obtained from lightly fixed (<4% PFA), and rapidly frozen tissues, which offers a key advantage of antigen preservation in situ in tissue sections. Tissue morphology is not as well preserved as FFPE sections.
Figure legend
Cryosection imaging shows the Mucin-1 proteins in the luminal membrane of the epithelium lining the mucosa of the mouse stomach. Note that the anti-Mucin-1 antibody signals have very low background in the cytoplasm.
Figure legend
High-magnification view of cryosection showing the Mucin-1 protein localization in the mouse stomach epithelium.
Formalin-fixed paraffin embedded (FFPE) tissue sections preserve tissue morphology better than cryosections. However, because formalin (10% formaldehyde) extensively crosslinked proteins in tissue sections, antigen retrieval is required to reverse the crosslinking reaction and to allow antibody access to antigen proteins.
Figure legend
FFPE section imaging shows the Mucin-1 proteins in the luminal membrane of the epithelium lining the mucosa of the mouse stomach. Note that the anti-Mucin-1 antibody signals have higher background in the cytoplasm than those on cryosection.
Figure legend
High-magnification view of FFPE section showing the Mucin-1 protein localization in the mouse stomach epithelium.
Item Cat No.: 01101
Antibody: Rat Mucin-1 (MUC1 or CD227) Polyclonal Antibody
Concentration: 0.25 mg/ml purified IgG
Application: Validated by immunofluorescence labeling (1:100)
Reactivity: Human, mouse, rat
Read More"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."
Jerrold R. Turner, M.D., Ph.D.
"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."
Univ.-Prof. Jay Gopalakrishnan PhD
"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."
Julie Craft Van De Weghe, PhD
"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"
Divyesh Joshi, PhD
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