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11-beta-HSD1 (HSD11B1) antibody labeling of mouse brain

11-beta-HSD1 (HSD11B1) antibody labeling of mouse brain

11-beta-HSD1 proteins are expressed by the astrocytes in the hippocampus of mouse brain.

11-beta-HSD1 in the mouse kidney

11-beta-HSD1 proteins are expressed by the resident macrophages in the mouse kidney.

11-beta-HSD1 (HSD11B1) antibody

$155.00 – $435.00

Item Cat No.: 52041

Antibody: Rat 11-beta-HSD1 (HSD11B1) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-11-beta-HSD1 (HSD11B1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from human and rodent tissues.

Optional Blocking Peptide

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Anti-11-beta-HSD1 (HSD11B1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from human and rodent tissues.

11-beta-Hydroxysteroid dehydrogenase type 1 (HSD1), also known as cortisone reductase, is an NADPH-dependent enzyme that is encoded by the HSD11B1 gene in human. 11-beta-HSD1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. 11-beta-HSD1 is highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal region of mouse HSD11B1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 81.3% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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