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Anoctamin-1 (ANO1 or TMEM16A) antibody labeling of mouse brain

Anoctamin-1 (ANO1 or TMEM16A) antibody labeling of mouse brain

Anoctamin-1 proteins are found in the endothelium lining the arterioles in the cerebral cortex of the mouse brain.

Anoctamin-1 in the choroid plexus

Anoctamin-1 proteins are found in the endothelium lining the capillary of the choroid plexus in the mouse brain.

Anoctamin-1 (ANO1 or TMEM16A) (C-terminus) antibody

$155.00 – $435.00

Item Cat No.: 20530

Antibody: Rat Anoctamin-1 (ANO1 or TMEM16A) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Mouse, rat

Anti-Anoctamin-1 (ANO1 or TMEM16A) (C-terminus) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent tissues.

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Optional Blocking Peptide

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

Product price:$155.00
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Anti-Anoctamin-1 (ANO1 or TMEM16A) (C-terminus) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent tissues.

Anoctamin-1 (ANO1), also known as Transmembrane member 16A (TMEM16A), is a voltage-gated calcium-activated anion channel, that is encoded by the ANO1 gene in human. Anoctamin-1 is expressed in the gastrointestinal tract and is highly expressed in interstitial cells of Cajal, where it plays an important role in pacemaker activity, neurotransduction of enteric motor neurotransmitters and regulation of gastrointestinal motility.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (14-aa) derived from the C-terminal cytoplasmic region of mouse Anoctamin-1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 64.3% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!

Annette Ehrhardt, PhD

Dept. of Pediatrics | Emory University

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