Protocol for Human iPS Cell Culture

Matrigel coating of culture dish Expand 1. Thaw Ready-to-use Matrigel on ice. Coat dish (35-mm to 150-mm diameter) by adding 500μl – 5ml ready-to-use gelatin solution to cover the bottom; rock the dish back and forth to let solution cover homogeneously. 2. Sit at room temperature for 1hr (keep sterile in culture hood). 3. Aspirate solution and […]

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