Hybridoma generation is a critical step on the path towards monoclonal antibody discovery for diagnostic and research applications. Developing hybridomas involves many complex steps that begin with injecting an animal (e.g. mouse or rat) with antigen to generate an immune response. Primary B cells harvested from the spleen or lymph nodes are fused with myeloma cells to generate hybridomas that are capable of secreting antibodies in vitro. Monoclonal selection of each stable hybridoma cell line establishes the basis of monoclonality of the antibodies that are discovered and manufactured from the hybridoma cells.

BiCell-mAb Generation Kit contains media and reagents for all steps of hybridoma generation and monoclonal antibody production. This kit has been validated for use of mouse and rat hybridoma development.