BiCell-mAb Medium PEG
$215.00
1 ml size
BiCell-mAb Medium PEG is a serum-free culture medium for inducing fusion of myeloma cells with B cells to produce hybridomas. This medium can generate >1 stable hybridoma cell clone per 105 fused myeloma cells.
This medium has been validated for use of mouse and rat hybridoma development.
This medium is part of BiCell-mAb Generation Kit (Cat No.: BCMABK0).
The medium contains:
Storage condition: 4°C
For Research Use Only. Not for use in clinical diagnostics.
Preparation of Myeloma Cells Expand Prewarm all culture media to 37oC. Thaw the parental myeloma cells (Sp2/0-Ag14 or X63-Ag8.653) from liquid nitrogen and culture in BiCell-mAb Medium MY for 3-5 days to fully recover cell growth to reach 1 x 106/ml cell density. Split cells at 1:5 into fresh BiCell-mAb Medium MY and let grow […]
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