Shop Antibodies
Best Seller

BiCellFectamaxVivo Transfection Reagent

$795.00$4,850.00

Item Cat No.: T0011

Application: In vivo transfection

Reactivity: Mouse, Rat

BiCellFectamaxVivo Transfection Reagent is a validated lipid nanoparticle (LNP) transfection tool able to deliver siRNA, mRNA, and DNA to a variety of internal organs in live animals via systemic or local injection routes.

Liposome is a common transfection approach used to carry DNA or RNA across the cell membrane by lipofection. Liposome entraps the transfected payload, e.g. DNA or RNA in an aqueous environment.

Liposome used for in vivo transfection is termed lipid nanoparticle (LNP). LNP includes four types of molecules: an ionizable amino lipid to bind nucleic acids, an amphipathic phospholipid to promote fusion with the plasma membranes, cholesterol to facilitate LNP stability and polyethylene glycol (PEG) lipid to improve colloidal stability and reduce reticuloendothelial clearance.

BiCellFectamaxVivo Transfection Reagent utilizes a mixture of ionizable cationic lipids 4A3-SC8 and DLin-MC3-DMA, phospholipids DOPE and DSPC, cholesterol, DMG conjugated PEG molecule to form stable LNPs and deliver siRNA, mRNA, and DNA to a variety of internal organs.

Sample type: siRNA, mRNA, and DNA

Injection route: systemic or local

Purpose of use: In vivo transfection

Storage buffer: lipid-aqueous solution

Storage condition: 4°C


For Research Use Only. Not for use in clinical diagnostics.

BiCell Scientific recommends using isotonic 5% glucose (sterile) as solvent for in vivo injection.

BiCell Scientific recommends using a ratio of nucleic acid to LNP (N/L) at 6-8 (w/v) according to the following table.

Amount of DNA or RNA (μg) Volume (μl) of LNP (N/L=6) Volume (μl) of LNP (N/L=8)
1 0.12 0.16
5 0.6 0.8
10 1.2 1.6
40 4.8 6.4
50 6 8
100 12 16

 

BiCell Scientific recommends the following DNA or RNA dosage at N/L ratio = 6 for common injection routes in mouse and rat.

Animal Route of injection Recommendation Nucleic acid dosage Injection volume
Mouse IV-Tail vein/retro-orbital 50 μg nucleic acid & 8.3 μl LNP reagent 40 – 60 μg 100 – 200 μl
IP 100 μg nucleic acid & 16 μl LNP reagent 100 – 200 μg 500 μl
Intratumoral 10 μg nucleic acid & 1.6 μl LNP reagent 5 – 15 μg 20 – 100 μl
Subcutaneous 5 μg nucleic acid & 0.8 μl LNP reagent 3 – 5 μg 5 – 15 μl
Intracerebral 5 μg nucleic acid & 0.8 μl LNP reagent 3 – 5 μg 5 – 15 μl
Rat IV-Tail vein/retro-orbital 150 μg nucleic acid & 25 μl LNP reagent 100 – 300 μg 500 -1000 μl
Intracerebral 5 μg nucleic acid & 0.8 μl LNP reagent 3 – 5 μg 5 – 15 μl

 

Protocol is given to demonstrate IV injection in mouse. Injection via other routes can be scaled up or down accordingly.

Preparation of 200 μl injection volume of 5 % glucose containing 50 μg of plasmid DNA and BiCellFectamaxVivo at N/L = 6

1. Dilute 50 μg of DNA into 50 μl H2O, add 50 μl 10% glucose and vortex gently.

2. Dilute 8.3 μl of BiCellFectamaxVivo into 41.7 μl H2O, add 50 μl of 10% glucose and vortex gently.

3. Add diluted BiCellFectamaxVivo to diluted DNA and vortex gently (volume: 200 μl; glucose content: 5%).

4. Incubate for 20 minutes at room temperature. 

5. Perform injections into animals via IV. 

Additional information

Volume

0.5 ml, 1 ml, 2 ml, 5 ml, 10 ml

Reviews

There are no reviews yet.

Be the first to review “BiCellFectamaxVivo Transfection Reagent”

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

Contact us for questions or custom requests!