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BiCellVivo mouse isotype control antibody


Item Cat No.: BCVI00

Antibody: Monoclonal Antibody

Concentration: 0.50 mg/ml

Application: In vivo

Reactivity: Mouse (strain dependent)

BiCellVivo mouse isotype control antibody is a recombinant mouse immunoglobulin (Ig) G(1/2a/2b/3)-kappa monoclonal antibody against trinitrophenol (TNP) hapten.

Monoclonal antibodies are able to block ligand-receptor bindings and induce antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) in vivo. In ADCC, the Fc region of an antibody binds to Fc receptors (FcγRs) on the surface of immune effector cells such as natural killers and macrophages, leading to phagocytosis or lysis of the targeted cells. In CDC, the antibodies kill the targeted cells by triggering the complement cascade at the cell surface. The use of xenogeneic antibodies, i.e., the monoclonal antibodies derived from human or rat, may cause severe CDC effects in various mouse models.

Mouse cells express different types of Fc receptors, e.g. FcγRI, FcγRII, FcγRIII, and FcγRV, which show different binding affinities to immunoglobulin (Ig) G subclasses (vide infra).  Therefore, one isotype of IgG molecule may only target a group of cells that express the cognate Fc receptor in the cell membrane. IgG subclasses exert different levels of effector functions of ADCC in the increasing order of IgG1 < IgG3 < IgG2a ≈ IgG2b. The induction of systemic ADCC effects may require the infusion of a mixture of IgG isotypes.

Fc receptor type FcγRI FcγRII FcγRIII FcγRV
Cellular expression T cell +
B cell +
NK cell +
Neutrophil + + +
Dendritic cell + + + +
Binding affinity IgG1 ++ +
IgG2a +++ + + ++++
IgG2b + ++ + ++++
IgG3 +

BiCellVivo mouse isotype control antibody is a recombinant antibody consisting in the Fv domain from the mouse anti-trinitrophenol antibody (clone SP6) and the Fc domain from each of the four subclasses of mouse IgG protein, IgG1, IgG2a, IgG2b, and IgG3. BiCellVivo mouse isotype control antibody is recommended to use as negative control for a specific subclass of IgG molecule.

Host/Isotype: Mouse/IgG1,2a,2b,3

Class: Monoclonal

Immunogen: trinitrophenol

Production platform: CHO cells

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics or therapeutics.

Additional information

Antibody Production Scale

100 ug, 1 mg, 10 mg


IgG1, IgG2a, IgG2b, IgG3


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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