Shop Antibodies

Demonstration of Bioreactor Scale-up Service

Left panel: spinner bioreactor system with capacity of continuous cultivation. Right panel: comparison of antibody production yield in bioreactor versus flask.

Best Seller

Bioreactor Scale-up of Antibody Production

$950.00 – $17,500.00

Antibody: Monoclonal Antibody

Concentration: 0.25 mg/ml of purified IgG

Application: In vitro and In vivo

Reactivity: Human, Mouse, Rat

BiCell Scientific Inc has developed a bioreactor culturing approach and a suitable serum free culture medium for hybridoma cells derived from mouse, rat and rabbit.

BiCell Scientific Inc has optimized hybridoma cell growth for monoclonal antibody secretion in continuous cultivation bioreactor with a chemically defined, serum-free culture medium (Cat No: BCMABK6). Continuous cultivation bioreactor permits monoclonal antibody secretion rate to reach 100-150 μg/ml medium, which is approximately 3-fold higher than that in adherent or suspension flasks.

Bioreactor Scale-up Service of Antibody Production can be used in conjunction with BiCell Scientific’s Transgenic Antibody Production Service (Cat No: BCFTIG), if customers have antibody sequences but not readily made hybridoma cells. Bioreactor Scale-up Service of Antibody Production includes affinity chromatography purification based upon Protein A, Protein G or Protein L column.

Bioreactor Workflow Chart

Steps Timeline (weeks) Deliverables/Internal testing milestone QC standard
Phase I: Hybridoma cell recovery

(hybridoma cells will be recovered from liquid nitrogen and cultured in serum containing medium to assess viability)

 1 week / COA

 

 

 

 
Phase II: Hybridoma cell adaptation to bioreactor

(hybridoma cells will be inoculated into serum-free medium and cultured in bioreactor to assess growth curve)

 

 

 2 weeks / COA
Phase III: Hybridoma cell expansion in bioreactor

(continuous cultivation is maintained in bioreactor with 20% medium removed and replenished on daily basis after log growth phase is reached)

 

 

 2 weeks 100 μl medium from daily removal ELISA
Phase IV: Affinity Chromatography

(culture medium will be centrifuged to remove hybridoma cells; supernatant will be purified with affinity chromatography to elute antibody proteins)

 

 1-2 weeks 1 mg to 1 g purified antibody ELISA

 

For Research Use Only. Not for use in clinical diagnostics.

Additional information

Antibody Production Scale

1 mg, 5 mg, 10 mg, 100 mg, 1 g
Host Species

Mouse, Rat, Rabbit

Reviews

There are no reviews yet.

Be the first to review “Bioreactor Scale-up of Antibody Production”

Related products

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

Contact us for questions or custom requests!

Email Us