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BIP (HSPA5 or GRP78) antibody labeling of mouse kidney

BIP (HSPA5 or GRP78) antibody labeling of mouse kidney

BIP proteins are found in the lumen of the endoplasmic reticulum in the epithelium making the distal tubules in the inner medulla of the mouse kidney.

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BIP (HSPA5 or GRP78) antibody

$155.00 – $435.00

Item Cat No.: 54020

Antibody: Rat BIP (HSPA5 or GRP78) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-BIP (HSPA5 or GRP78) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

Product price: $155.00
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Anti-BIP (HSPA5 or GRP78) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Binding immunoglobulin protein (BiP) also known as (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a endoplasmic reticulum (ER) protein that is encoded by the HSPA5 gene in human. BIP is a member of the heat shock protein 70 (HSP70) family that is involved in the folding and assembly of proteins in the ER. BIP is located in the lumen of the ER that binds newly synthesized proteins as they are translocated into the ER.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (13-aa) derived from the C-terminal region of mouse BIP protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 92.3% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

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Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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