Cadherin-6 (zebrafish) antibody labeling of zebrafish embryo

Cadherin-6 proteins are found in the cell junctions of 18 hpf embryo fixed by cold methanol. (Courtesy of Dr. Moe Fukumoto, National Cerebral and Cardiovascular Center Research Institute, Japan)

Cadherin-6 (CDH6) (zebrafish) antibody

Name: Cadherin-6 (CDH6) (zebrafish) antibody
SKU: 00106z
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00106z

Antibody: Rat Cadherin-6 (zebrafish) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Zebrafish

Anti-Cadherin-6 (zebrafish) antibody is validated on zebrafish tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from zebrafish tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-Cadherin-6 (zebrafish) antibody is validated on zebrafish tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from zebrafish tissues.

Cadherin-6 is a a member of the cadherin superfamily, which is encoded by the CDH6 gene in zebrafish. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. Cadherin-6 is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (17-aa) derived from the N-terminal extracellular region of zebrafish cadherin-6 protein

Species homology of immunogen: Synthetic peptide sequence showing 70.6% homology to human sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Cadherin

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Cadherin-6 (CDH6) (zebrafish) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Cadherin

Reviews

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