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CAR-T Transfection Virus (mCD19-1D3-scFv-CD28-CD3)


Item Cat No.: BCAR01

Application: CAR-T cell development

Reactivity: Cell culture

BiCell Scientific’s CAR-T transfection virus is able to infect primary T cells to express the chimeric antigen receptor of mCD19-1D3-scFv-CD28-CD3.

1 ml of concentrated virus at titer of 1 x 10IFU /ml

Chimeric antigen receptor T cells (also known as CAR T cells) are T cells that have been genetically engineered to produce an artificial T cell receptor for use in immunotherapy.

The extracellular domain of CAR consists in the single chain variable region moiety (scFv) from a monoclonal antibody (clone: 1D3; Rat IgG2a) raised against mouse CD19 protein. The cytoplasmic domain of CAR consists in a portion of the murine CD28 molecule from amino acids IEFMY to the 3′- terminus and the cytoplasmic region of the murine CD3ζ molecule from amino acids RAKFS to the 3′-terminus.

BiCell Scientific’s CAR-T transfection virus is used to deliver the chimeric antigen receptor of mCD19-1D3-scFv-CD28-CD3 into primary T cells that are cultured in vitro or ex vivo. The CAR-T transfection virus also expresses the GFP marker protein to facilitate FACS sorting applications.

Class: Retrovirus/Lentivirus pseudotyped with VSV-G coat protein

Antigen: mouse CD19 protein

Marker protein: GFP

Purification: Ultracentrifugation

Storage buffer: PBS

Storage condition: –80°C

For Research Use Only. Not for use in clinical diagnostics.

Additional information

Type of virus

Retrovirus, Lentivirus

CD3 activation option

CD3-intact-ITAM, CD3-attenuated-ITAM


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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