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Centrin-1 antibody

$99.00$295.00

Item Cat No.: 01061

Antibody: Rabbit Centrin-1 Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

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Optional Blocking Peptide

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Anti-Centrin-1 antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Centrin-1 (CETN1) is encoded by the CETN1 gene in human. It belongs to the Centrin family of proteins. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing during the cell cycle. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (18-aa) derived from the C-terminal region of mouse Centrin-1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 94.4% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Centrosome (embryo section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Blocking buffer Expand NaCl 8 g KCl 0.2 g […]

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Protocol for Staining Centrosome (retina section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 g […]

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