CHRNA3 (NACHRA3) antibody

$155.00 – $435.00

Item Cat No.: 15183

Antibody: Rabbit CHRNA3 (NACHRA3) polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, Mouse, Rat

Anti-CHRNA3 (NACHRA3) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Cholinergic receptor nicotinic alpha 3 subunit (CHRNA3) is encoded by the CHRNA3 gene in human. Nicotinic acetylcholine receptors are receptor proteins that respond to the neurotransmitter acetylcholine. Nicotinic receptors also respond to drugs such as the agonist nicotine. They are found in the central and peripheral nervous system, muscle, and many other tissues of many organisms.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (14-aa) derived from the N-terminal extracellular region of mouse CHRNA3 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 78.6% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining G-Protein Coupled Receptor

Name: CHRNA3 (NACHRA3) antibody
SKU: 15183
Availability: InStock

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]

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CHRNA3 (NACHRA3) antibody

Optional Blocking Peptide

Protocol for Staining G-Protein Coupled Receptor

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