CHRNA3 (NACHRA3) antibody labeling of mouse cerebral cortex

CHRNA3 proteins are found in the neurons and their dendrites in the cortex of mouse cerebrum.

CHRNA3 in the hippocampus

CHRNA3 proteins are found in the hippocampal neurons and their dendrites of the mouse cerebrum.

CHRNA3 in the cerebellum

CHRNA3 proteins are found in the Purkinje cells and their dendrites of the mouse cerebellum. Mol., molecular layer; Gr., granular layer.

CHRNA3 (NACHRA3) antibody

Name: CHRNA3 (NACHRA3) antibody
SKU: 15183
Availability: InStock

$155.00 – $435.00

Item Cat No.: 15183

Antibody: Rabbit CHRNA3 (NACHRA3) polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, Mouse, Rat

Anti-CHRNA3 (NACHRA3) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-CHRNA3 (NACHRA3) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Cholinergic receptor nicotinic alpha 3 subunit (CHRNA3) is encoded by the CHRNA3 gene in human. Nicotinic acetylcholine receptors are receptor proteins that respond to the neurotransmitter acetylcholine. Nicotinic receptors also respond to drugs such as the agonist nicotine. They are found in the central and peripheral nervous system, muscle, and many other tissues of many organisms.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (14-aa) derived from the N-terminal extracellular region of mouse CHRNA3 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 78.6% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining G-Protein Coupled Receptor

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]

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CHRNA3 (NACHRA3) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining G-Protein Coupled Receptor

Reviews

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