Anti-Claudin-18 (CLDN18) antibody labeling of mouse tissue

Claudin-18 (CLDN18) antibody labeling of the stomach

Claudin-18 proteins are found in the basolateral membrane of the epithelial layer in the mouse stomach. Tight junction are labeled with anti-ZO-1 antibody.

Claudin-18 in the mouse lung

Claudin-18 proteins are found in the tight junctions of the epithelium making the alveolar sacs (A.S.). Note that the epithelium in the bronchiole (B.) lacks Claudin-18 protein expression.

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Claudin-18 (CLDN18) antibody

Name: Claudin-18 (CLDN18) antibody
SKU: 00218
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00218

Antibody: Rabbit Anti-Claudin-18 (CLDN18) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-Claudin-18 (CLDN18) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-Claudin-18 (CLDN18) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Claudin-18 is a protein that is encoded by the CLDN18 gene in human. Claudin-18 belongs to the Claudin protein superfamily.

Claudins are tetraspan proteins making the tight junction. Claudins have four transmembrane domains, two extracellular loops (ECLs: ECL1 and ECL2), amino- and carboxyl-terminal cytoplasmic domains, and a short cytoplasmic motif.

Claudin-18 has two splicing isoforms: 18.a1 and 18.a2. The difference is within the ECL1 domain. CLDN18.a1 is expressed in the lung and CLDN18.a2 is expressed in the stomach. In particular, CLDN18.a2 has been implicated in gastric cancer progression. Monoclonal antibodies against CLDN18.a2 is under clinical trial to treat advanced gastric cancer.

This antibody is able to recognize both CLDN18.a1 and CLDN18.a2.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (14-aa) derived from the C-terminal region of mouse Claudin-18.a1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 78.5% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Claudin

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 g KCl […]

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Claudin-18 (CLDN18) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Claudin

Reviews

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