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Crispr Knockout & Knockin in Somatic Cells


Item Cat No.: BCCRSM

Application: Genome editing

Reactivity: Human, mouse, rat cells

BiCell Scientific’s Crispr KO & KI service in somatic cells utilizes the Crispr-Cas9 system to edit single or double alleles in the mammalian genome to achieve loss-of-gene function, gain-of-gain function, insertion of genetic materials and replacement with human DNA sequences.

CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms. Cas9 (or “CRISPR-associated protein 9”) is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes in mammalian cells.

CRISPR genome editing in somatic cells is more time-consuming than genome editing in ES cells, because both alleles of a gene are often needed to be altered. Without the help of mouse breeding, the traits of genetic alteration can only be screened but not segregated.

BiCell Scientific’s Crispr KO & KI service in somatic cells guarantees success of genetic alteration as well as phenotype validation. The genetically edited allele will be deep-sequenced to confirm the intended modification.

  • Molecular cloning of gRNA into pX330-U6-hSpCas9 vector.
  • Molecular cloning of targeting vector carrying intended mutation or insertion (KI projects only).
  • Electroporation of mammalian somatic cells of customer’s parental line with pX330-U6-hSpCas9 and targeting vector.
  • Perform Surveyor nuclease digestion (for KO projects) or qPCR (for KI projects) to identify positive clones.
  • Deep-sequencing positive clones to identify intended modification.

Customers will receive the following deliverables at the end of each project:

  • One frozen mammalian somatic cell clone with intended genetic modification (1 × 106 cells)
  • Deep-sequencing results

(Customers select the clone of their interest. BiCell Scientific Inc will bank the remaining clones. Additional clones are offered at $950 each).

For Research Use Only. Not for use in clinical diagnostics or therapeutics.

  • Molecular cloning of gRNA into pX330-U6-hSpCas9 vector and targeting vector – 2-3 weeks
  • Electroporation of mammalian somatic cells – 1-2 weeks
  • Cell culture and clonal selection – 3-4 weeks
  • Surveyor nuclease test or qPCR test to shortlist positive clones – 2-3 weeks
  • Deep sequencing of shortlisted clones – 1-2 weeks

Additional information

Service type

KO, KI-point mutation, KI-reporter, Humanized-allele


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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