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Custom Adeno-Associated Virus Production

$3,150.00

Item Cat No.: BCAAV1

Application: Custom Adeno-Associated Virus Production

Reactivity: Cell culture, animal

BiCell Scientific’s Adeno-Associated Virus (AAV) production service provides customized AAV design and packaging services including AAV vector cloning, AAV generation, packaging and titration, target cell transduction and transgenic expression validation.

Adeno-Associated Virus (AAV) is a non-enveloped virus that belongs to the Parvovirus family of the Dependovirus genus. The AAV genome is a linear, single-strand DNA molecule of approximately 4.7 kb that has terminal hairpin structures called inverted terminal repeats (ITRs) at both ends. ITRs function as origins of genomic replication and contribute to packaging of viral particles.

AAV can be used to transduce genes into both proliferating and non-proliferating cells and can impart long-term expression in non-dividing cells. In addition, AAV doesn’t integrate into the genome of host cells or has little immunogenicity, so is suitable for the transduction of genes into animals.

There are more than 100 serotypes of AAV, and the host specificity and characteristics of the virus differ among serotypes. BiCell Scientific’s AAV service utilizes the Cap protein of serotype 1 (AAV1) that has high transduction efficiency for muscle, liver, respiratory tract, and central nervous system.

  • Molecular cloning of transgene into AAV vector
  • Transfection of HEK293 cells with AAV vector as well as vectors expressing Rep, Cap, E2A, E4, VA
  • Harvesting and purification of AAV from cell lysate
  • Titrating virus titer with realtime PCR
  • Transduction and validation of transgene expression in target cells with IF/IHC

Customers will receive the following deliverables at the end of each project:

  • Validated AAV backbone vector containing desired transgene
  • Validated pre-packaged AAV of 1ml at titer of 1×109/ml (serotype 1)
  • Validation of transgenic expression in target cells with IF/IHC
  • Validation of target cell transduction at >90% with transgene expression
  • AAV vector cloning – 2-3 weeks
  • AAV packaging  – 1-2 weeks
  • Target cell transduction – 1-2 weeks
  • Transgenic expression validation – 2-3 weeks

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

Contact us for questions or custom requests!