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Custom Adenovirus Production

$3,150.00

Item Cat No.: BCFTAV

Application: Custom Adenovirus Production

Reactivity: Cell culture, animal

BiCell Scientific’s adenovirus production service provides customized adenovirus design and packaging services including adenovirus vector cloning, adenovirus generation, packaging and titration, target cell transduction and transgenic expression validation.

Adenoviruses (members of the family Adenoviridae) are medium-sized (90–100 nm), nonenveloped (without an outer lipid bilayer) viruses with an icosahedral nucleocapsid containing a double-stranded DNA genome. Adenoviruses can be used to transduce genes into both proliferating and non-proliferating cells and can impart long-term expression in non-dividing cells.

Because adenovirus doesn’t integrate into host genome, it provides a safe and transient transduction means of gene therapy. BiCell Scientific’s adenovirus service allows the transduction and expression of transgene up to 8kb in size in host cells. BiCell Scientific’s adenovirus is replication incompetent due to the deletion of E1 and E3 genes from the viral genome.

  • Molecular cloning of transgene into adenovirus vector
  • Transfection of HEK293-E1A/E1B cells with adenovirus vector
  • Harvesting and purification of adenovirus from cell lysate
  • Titrating virus titer with realtime PCR
  • Transduction and validation of transgene expression in target cells with IF/IHC

Customers will receive the following deliverables at the end of each project:

  • Validated adenovirus backbone vector containing desired transgene
  • Validated pre-packaged adenovirus of 1ml at titer of 1×109 IFU/ml
  • Validation of transgenic expression in target cells with IF/IHC
  • Validation of target cell transduction at >90% with transgene expression
  • Adenovirus vector cloning – 2-3 weeks
  • Adenovirus packaging  – 1-2 weeks
  • Target cell transduction – 1-2 weeks
  • Transgenic expression validation – 2-3 weeks

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

Contact us for questions or custom requests!