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Custom Antibody Array

$895.00$2,685.00

Antibody: Polyclonal and Monoclonal Antibody

Concentration: 0.25 mg/ml of purified IgG

Application: Proteomics

Reactivity: Human, Mouse, Rat

BiCell Scientific Inc has developed Western blot-specific recognition antibodies and microprinting techniques to manufacture antibody arrays on glass slides to permit detection and quantitation of sample proteins under denaturing condition.

1 set (2 slides)

Antibody array allows detecting multiple protein expression simultaneously, offering a proteomic view of vital cellular functions. The antibodies used for arrays, however, are problematic and inconsistent. The antibodies raised against naturally folded proteins are not suited for array studies, largely because arrays, just like Western blots, require target proteins to be denatured and linearized to establish concentration-to-binding linearity.

BiCell Scientific Inc is able to raise polyclonal and monoclonal antibodies against unfolded target epitope. Such antibodies can dramatically improve the binding specificity on Western blot, which are vital tools of quantitative measurements of protein expression in endogenous cell or tissue samples. BiCell Scientific Inc can now microprint Western blot specific antibodies onto glass slides to make antibody arrays suited for proteomic analyses.

  • Antibody array glass slide with removable chamber
  • Cell lysis buffer (SDS, 2-Mercaptoethanol, NaCl, Phosphate buffer) (10 ml)
  • Sulfo-NHS-LC-Biotin (10 mM)
  • Biotinylation stop solution (Glycine, Tris buffer)
  • Streptavidin-HRP (50 μl)
  • Block buffer (BSA, Tween-20, NaCl, Tris buffer) (50 ml)
  • Wash buffer (Tween-20, NaCl, Tris buffer) (50 ml)

For Research Use Only. Not for use in clinical diagnostics.

  • Collect tissues or pellet cells by centrifugation.
  • Add Cell lysis buffer to tissue or cell pellet at 10:1 volume.
  • Homogenize tissue or cell pellet by pipetting and rotating at 4oC for 30min.
  • Centrifuge the sample at 10,000 xg at 4oC for 10min.
  • Collect the supernatant and measure total protein content at OD280.
  • Remove 1-10 μg of total protein and add Cell lysis buffer to make volume to 100 μl.
  • Heat the sample at 95oC for 5min to denature all proteins and chill on ice for 5min.
  • Add 2 μl of 10 mM Sulfo-NHS-LC-Biotin per 100 μl of sample volume. Rotate at room temperature for 1hr.
  • Add 10 μl of Biotinylation stop solution per 100 μl of sample volume.
  • Add 400 μl of Block buffer per 100 μl of sample volume and transfer all to a glass slide chamber.
  • Incubate the sample with the glass slide at room temperature for 1hr.
  • Aspirate the sample and wash the glass slide with Wash buffer 3 times.
  • Add 5 μl of Streptavidin-HRP to 500 μl of Block buffer and transfer all to the glass slide chamber.
  • Incubate Streptavidin-HRP with the glass slide at room temperature for 1hr.
  • Aspirate Streptavidin-HRP and wash the glass slide with Wash buffer 3 times.
  • Add ECL or other chemiluminescent substrate solution.
  • Image the glass slide with ChemiDoc, iBright® or Azure Imagers.

Customers can elect any BiCell Scientific-made Western blot-specific antibody to be printed onto antibody array. Customers please type the antibody list onto the purchase order or email the antibody list to us.

tech@bicellscientific.com

Additional information

Array format

24 antibodies, 64 antibodies, 96 antibodies

Target species

Human, Mouse, Rat

Reviews

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Be the first to review “Custom Antibody Array”

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

Contact us for questions or custom requests!