Name: Custom Antibody Array
SKU: 6920
Availability: InStock

Custom Antibody Array

$895.00 – $2,685.00

Antibody: Polyclonal and Monoclonal Antibody

Concentration: 0.25 mg/ml of purified IgG

Application: Proteomics

Reactivity: Human, Mouse, Rat

BiCell Scientific Inc has developed Western blot-specific recognition antibodies and microprinting techniques to manufacture antibody arrays on glass slides to permit detection and quantitation of sample proteins under denaturing condition.

1 set (2 slides)

Antibody array allows detecting multiple protein expression simultaneously, offering a proteomic view of vital cellular functions. The antibodies used for arrays, however, are problematic and inconsistent. The antibodies raised against naturally folded proteins are not suited for array studies, largely because arrays, just like Western blots, require target proteins to be denatured and linearized to establish concentration-to-binding linearity.

BiCell Scientific Inc is able to raise polyclonal and monoclonal antibodies against unfolded target epitope. Such antibodies can dramatically improve the binding specificity on Western blot, which are vital tools of quantitative measurements of protein expression in endogenous cell or tissue samples. BiCell Scientific Inc can now microprint Western blot specific antibodies onto glass slides to make antibody arrays suited for proteomic analyses.

Antibody array glass slide with removable chamber
Cell lysis buffer (SDS, 2-Mercaptoethanol, NaCl, Phosphate buffer) (10 ml)
Sulfo-NHS-LC-Biotin (10 mM)
Biotinylation stop solution (Glycine, Tris buffer)
Streptavidin-HRP (50 μl)
Block buffer (BSA, Tween-20, NaCl, Tris buffer) (50 ml)
Wash buffer (Tween-20, NaCl, Tris buffer) (50 ml)

For Research Use Only. Not for use in clinical diagnostics.

Collect tissues or pellet cells by centrifugation.
Add Cell lysis buffer to tissue or cell pellet at 10:1 volume.
Homogenize tissue or cell pellet by pipetting and rotating at 4^oC for 30min.
Centrifuge the sample at 10,000 xg at 4^oC for 10min.
Collect the supernatant and measure total protein content at OD₂₈₀.
Remove 1-10 μg of total protein and add Cell lysis buffer to make volume to 100 μl.
Heat the sample at 95^oC for 5min to denature all proteins and chill on ice for 5min.
Add 2 μl of 10 mM Sulfo-NHS-LC-Biotin per 100 μl of sample volume. Rotate at room temperature for 1hr.
Add 10 μl of Biotinylation stop solution per 100 μl of sample volume.
Add 400 μl of Block buffer per 100 μl of sample volume and transfer all to a glass slide chamber.
Incubate the sample with the glass slide at room temperature for 1hr.
Aspirate the sample and wash the glass slide with Wash buffer 3 times.
Add 5 μl of Streptavidin-HRP to 500 μl of Block buffer and transfer all to the glass slide chamber.
Incubate Streptavidin-HRP with the glass slide at room temperature for 1hr.
Aspirate Streptavidin-HRP and wash the glass slide with Wash buffer 3 times.
Add ECL or other chemiluminescent substrate solution.
Image the glass slide with ChemiDoc^™, iBright^® or Azure Imagers.

Customers can elect any BiCell Scientific-made Western blot-specific antibody to be printed onto antibody array. Customers please type the antibody list onto the purchase order or email the antibody list to us.

tech@bicellscientific.com

Additional information

Array format	24 antibodies, 64 antibodies, 96 antibodies
Target species	Human, Mouse, Rat

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