Histologic stain and immunofluorescence

Top panel demonstrates the technique of histologic staining on FFPE sections of the mouse kidney. H/E stains the cytoplasm and the nucleus; PAS stains the brush border of the proximal tubule. CD: collecting duct; TAL: thick ascending limb. Bottom panel demonstrates the technique of immunofluorescent labeling on cryosections of the mouse kidney. Aqp2 antibody labels the principal cells of the collecting duct; Pendrin antibody labels the intercalated cells of the collecting duct.

Fluorescent IHC on mouse FFPE section

FFPE section of mouse kidney was deparaffinized followed by antigen retrieval and IHC with anti-Aqp2 antibody and TRITC labeled secondary antibody.

Fluorescent IHC on human FFPE section

FFPE section of human kidney was deparaffinized followed by antigen retrieval and IHC with anti-Aqp2 antibody and TRITC labeled secondary antibody.

Dual-color fluorescent IHC on FFPE section

FFPE section of mouse kidney was immunostained by anti-Calbindin and anti-Pendrin antibodies simultaneously and imaged by two fluorophores. Note that Calbindin antibody labels the cytoplasm of the principal cells in the DCT2 tubules, while Pendrin antibody labels the luminal membrane of the intercalated cells in the DCT2 and collecting duct tubules.

Low-magnification view of FFPE section labeled with two fluorophores

FFPE sections are of better morphology than cryosections. FFPE sections can be labeled with dual-color fluorophores as efficiently as cryosections.

TUNEL Stain on FFPE section

Fluorescent TUNEL stain was performed on FFPE section of rat liver. PV: portal vein; CV: central vein.

MTOR in mouse small intestine

MTOR proteins are found in the luminal membrane as well as in the intracellular vesicles of the epithelium from the mouse small intestine of FFPE section.

E-cadherin in mouse small intestine (base)

E-cadherin proteins are found in the cell junctions of the epithelium from the mouse small intestine of FFPE section.

E-cadherin in mouse small intestine (villus)

E-cadherin proteins are found in the cell junctions of the epithelium from the mouse small intestine of FFPE section.

In situ hybridization for Col1a1 mRNA in mouse bladder

Col1a1 mRNA expression is found in the lamina propria of normal mouse bladder as well as in mouse bladder with outlet obstruction disease. Epi – epithelium; LP – lamina propria.

In situ hybridization for microRNA-29a in mouse bladder

MicroRNA-29a is highly expressed by the urothelium in the bladder of wild-type (WT) mouse but is absent in the bladder of microRNA-29a knockout (KO) mouse.

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Histopathology Service

Name: Histopathology Service
SKU: 5512
Availability: InStock

$49.00 – $119.00

Histopathology Service provides standard and customized research-specific histology and pathology services including routine and special histological stains as well as immunofluorescence and immunohistochemistry with BiCell Scientific antibodies and in situ hybridization against mRNA or microRNA.

Service Type

Clear

Optional Digital Imaging Add-on

Add digital imaging using Nikon Ni-E motorized microscope (+$49.00)

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Histopathology Service provides standard and customized research-specific histology and pathology services including routine formalin fixation, and paraffin embedding and sectioning, and cryosectioning of frozen sections.

Histopathology Service offers routine and special histological stains, immunohistochemistry and immunofluorescence, and in situ hybridization against mRNA or microRNA, as well as digitized imaging data collection using Nikon Ni-E motorized microscope equipped with CCD fluorescence camera and bright-field color camera.

Histopathology Service has worked with a wide variety of tissues and staining techniques from mice, rats, non-human primates, pigs and humans and can develop protocols for new antibodies.

Tissue processing and embedding

Sectioning and staining of paraffin-embedded and cryostat sections

Routine and special histological stains (see “Sample Submission” for the list of stains)

Immunohistochemistry (DAB or other labels including fluorescent labels) (see “Sample Submission” to select an antibody from BiCell Scientific catalog)

Immunofluorescence (Alexa Fluor 488 or other colors) (see “Sample Submission” to select an antibody from BiCell Scientific catalog)

In situ hybridization (DIG probes against mRNA or microRNA) (see “Sample Submission” to select a mRNA or microRNA probe)

Digital imaging using Nikon Ni-E motorized microscope

Each sample will have 2 consecutive sections on one slide with specified stain or label. Slides will be examined with light microscopy or epifluorescence microscopy. Slides with meaningful signals will be shipped to customers. Imaged micrographs will be sent to customers via BiCell Blue Cloud.

Turnaround time

Small orders of 10 samples or less – 5 days
Large orders of greater than 10 samples -2 weeks

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Fixation and shipping instructions for Immunofluorescence service

1. Immunofluorescence is performed on cryosections. Tissue samples can be either fixed or freshly dissected, and rapidly frozen.

2. A fixative containing 1-4% paraformaldehyde is acceptable. In order to obtain optimal fixation, tissue slices should be < 2.0 x 2.0 centimeters in area with a thickness of < 3 millimeters. The volume of fixative must be > 10 times the volume of sample. Sample must be fixed for a minimum of 24hrs.

3. Tissues, either fixed or freshly dissected, must be frozen in a cryo-mold on dry ice or in -80oC freezer, and shipped in a foam box containing dry ice.

4. Please use FedEx overnight shipping service to send samples to us at the address below.

BiCell Scientific
11466 Schenk Drive
Maryland Heights, MO 63043
Phone: 314 818 9655

Fixation and shipping instructions for immunohistochemistry service

1. In order to obtain optimal fixation (and subsequent processing), tissue slices should be < 2.0 x 2.0 centimeters in area with a thickness of < 3 millimeters.

2. Either 10% formalin or 4% paraformaldehyde based fixative is acceptable for immunohistochemistry. The volume of fixative must be > 10 times the volume of sample. Sample must be fixed for a minimum of 24hrs.

3. Tissues can be submitted in fixative, but it is preferable that samples be transferred to 70% ethanol before submission and submitted in 70% ethanol. Please note the fluid type on the sample container.

4. Please submit samples in leak-proof flat bottom glass containers made for histology if possible. Other types of flat bottom containers are acceptable. Avoid containers with narrow bottoms, e.g. 15mL falcon tubes, as tissues at the bottom of the tube may not have sufficient space to be adequately fixed.

5. Please use FedEx overnight shipping service to send samples to us at the address below.

BiCell Scientific
11466 Schenk Drive
Maryland Heights, MO 63043
Phone: 314 818 9655

Fixation and shipping instructions for routine histology

1. In order to obtain optimal fixation (and subsequent processing), tissue slices should be < 2.0 x

2.0 centimeters in area with a thickness of < 3 millimeters.

2. Either 10% formalin or 4% paraformaldehyde fixative is acceptable. The volume of fixative must be > 10 times the volume of sample. Sample must be fixed for a minimum of 24hrs.

3. Tissues can be submitted in fixative, but it is preferable that samples be transferred to 70% ethanol before submission and submitted in 70% ethanol. Please note the fluid type on the sample container.

4. Please submit samples in leak-proof flat bottom glass containers made for histology if possible. Other types of flat bottom containers are acceptable. Avoid containers with narrow bottoms, e.g. 15mL falcon tubes, as tissues at the bottom of the tube may not have sufficient space to be adequately fixed.

5. Please use FedEx overnight shipping service to send samples to us at the address below.

BiCell Scientific
11466 Schenk Drive
Maryland Heights, MO 63043
Phone: 314 818 9655

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Additional information

Service Type	Histologic stain on FFPE (per sample), Immunofluorescence on cryosection (per sample), Immunohistochemistry on FFPE (per sample), In situ hybridization on FFPE (per sample)

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