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Histopathology Service

Name: Histopathology Service
SKU: 5512
Availability: InStock

$49.00 – $99.00

Histopathology Service provides standard and customized research-specific histology and pathology services including routine and special histological stains as well as immunofluorescence and immunohistochemistry with BiCell Scientific antibodies.

Service Type

Clear

Optional Digital Imaging Add-on

Add digital imaging using Nikon Ni-E motorized microscope + $49.00

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Histopathology Service provides standard and customized research-specific histology and pathology services including routine formalin fixation, and paraffin embedding and sectioning, and cryosectioning of frozen sections.

Histopathology Service offers routine and special histological stains, immunohistochemistry and immunofluorescence, as well as digitized imaging data collection using Nikon Ni-E motorized microscope equipped with CCD fluorescence camera and bright-field color camera.

Histopathology Service has worked with a wide variety of tissues and staining techniques from mice, rats, non-human primates, pigs and humans and can develop protocols for new antibodies.

Tissue processing and embedding

Sectioning and staining of paraffin-embedded and cryostat sections

Routine and special histological stains (see “Sample Submission” for the list of stains)

Immunohistochemistry (DAB or other labels including fluorescent labels) (see “Sample Submission” to select an antibody from BiCell Scientific catalog)

Immunofluorescence (Alexa Fluor 488 or other colors) (see “Sample Submission” to select an antibody from BiCell Scientific catalog)

Digital imaging using Nikon Ni-E motorized microscope

Each sample will have 2 consecutive sections on one slide with specified stain or label. Slides will be examined with light microscopy or epifluorescence microscopy. Slides with meaningful signals will be shipped to customers. Imaged micrographs will be sent to customers via Google Cloud.

Turnaround time

Small orders of 10 samples or less – 5 days
Large orders of greater than 10 samples -2 weeks

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Shipping Instructions

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Fixation and shipping instructions for Immunofluorescence service

1. Immunofluorescence is performed on cryosections. Tissue samples can be either fixed or freshly dissected, and rapidly frozen.

2. A fixative containing 1-4% paraformaldehyde is acceptable. In order to obtain optimal fixation, tissue slices should be < 2.0 x 2.0 centimeters in area with a thickness of < 3 millimeters. The volume of fixative must be > 10 times the volume of sample. Sample must be fixed for a minimum of 24hrs.

3. Tissues, either fixed or freshly dissected, must be frozen in a cryo-mold on dry ice or in -80oC freezer, and shipped in a foam box containing dry ice.

4. Please use FedEx overnight shipping service to send samples to us at the address below.

BiCell Scientific
11466 Schenk Drive
Maryland Heights, MO 63043
Phone: 314 818 9655

Fixation and shipping instructions for immunohistochemistry service

1. In order to obtain optimal fixation (and subsequent processing), tissue slices should be < 2.0 x 2.0 centimeters in area with a thickness of < 3 millimeters.

2. Either 10% formalin or 4% paraformaldehyde based fixative is acceptable for immunohistochemistry. The volume of fixative must be > 10 times the volume of sample. Sample must be fixed for a minimum of 24hrs.

3. Tissues can be submitted in fixative, but it is preferable that samples be transferred to 70% ethanol before submission and submitted in 70% ethanol. Please note the fluid type on the sample container.

4. Please submit samples in leak-proof flat bottom glass containers made for histology if possible. Other types of flat bottom containers are acceptable. Avoid containers with narrow bottoms, e.g. 15mL falcon tubes, as tissues at the bottom of the tube may not have sufficient space to be adequately fixed.

5. Please use FedEx overnight shipping service to send samples to us at the address below.

BiCell Scientific
11466 Schenk Drive
Maryland Heights, MO 63043
Phone: 314 818 9655

Fixation and shipping instructions for routine histology

1. In order to obtain optimal fixation (and subsequent processing), tissue slices should be < 2.0 x

2.0 centimeters in area with a thickness of < 3 millimeters.

2. Either 10% formalin or 4% paraformaldehyde fixative is acceptable. The volume of fixative must be > 10 times the volume of sample. Sample must be fixed for a minimum of 24hrs.

3. Tissues can be submitted in fixative, but it is preferable that samples be transferred to 70% ethanol before submission and submitted in 70% ethanol. Please note the fluid type on the sample container.

4. Please submit samples in leak-proof flat bottom glass containers made for histology if possible. Other types of flat bottom containers are acceptable. Avoid containers with narrow bottoms, e.g. 15mL falcon tubes, as tissues at the bottom of the tube may not have sufficient space to be adequately fixed.

5. Please use FedEx overnight shipping service to send samples to us at the address below.

BiCell Scientific
11466 Schenk Drive
Maryland Heights, MO 63043
Phone: 314 818 9655

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Additional information

Service Type	Histologic stain on FFPE (per sample), Immunofluorescence on cryosection (per sample), Immunohistochemistry on FFPE (per sample)

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