Perfusion and fixation of mouse tissues with paraformaldehyde

Mouse Anesthesia Expand 1.  Place each mouse into the induction chamber of VetEquip multiple-patient isoflurane vaporizer. 2.  Set oxygen flow rate to 500 cc/min; set isoflurane vaporizer dial to 2.5. 3.  Take one animal from the induction chamber and continue inducing anesthesia with the breathing circuit in a chemical fume hood. Figure legend Left panel: […]

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