ILDR2 (Angulin-3) antibody staining of mouse tissue

ILDR2 (Angulin-3) antibody staining of the embryonic intestine

ILDR2 proteins are found in the tricellular tight junctions in the small intestine from the embryonic mouse at E15.5.

ILDR2 in the embryonic kidney

ILDR2 proteins are found reinforced in the tricellular tight junctions of a subset of kidney tubules from an embryonic mouse at E18.5. The positive tubules appear to be the proximal tubule judged from the shape and arrangement of the nuclei.

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ILDR2 (Angulin-3) antibody

Name: ILDR2 (Angulin-3) antibody
SKU: 00303
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00303

Antibody: Rabbit ILDR2 (Angulin-3) polyclonal antibody

Concentration: 0.25 mg/ml

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-ILDR2 (Angulin-3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-ILDR2 (Angulin-3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

ILDR2, which stands for immunoglobulin [Ig]-like domain-containing receptor 2, also known as angulin-3, belongs to the angulin protein family, which makes the tricellular tight junction in epithelium or endothelium.

ILDR2 is encoded by the ILDR2 gene in human. ILDR2 has a single transmembrane domain, an amino-terminal extracellular Ig-like domain, and a carboxyl-terminal cytoplasmic domain.

ILDR2 is expressed by a variety of epithelial tissues in developing organ systems.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (13-aa) derived from the C-terminal intracellular region of human ILDR2 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Tricellular Junction Protein

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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ILDR2 (Angulin-3) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Tricellular Junction Protein

Reviews

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