JAM-A (F11R or CD321) antibody labeling of mouse tissue

JAM-A (F11R or CD321) antibody labeling of the developing intestine

JAM-A proteins are localized to the tight junctions (counter-stained by ZO-1) in the mucosal epithelium of the small intestine from an embryonic mouse at E15.5.

JAM-A in the developing lung

JAM-A proteins are localized to the tight junctions (counter-stained with ZO-1) in the lung from an embryonic mouse at E15.5. B: bronchiole; AS: alveolar sac.

JAM-A (F11R or CD321) antibody

Name: JAM-A (F11R or CD321) antibody
SKU: 00251
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00251

Antibody: Rabbit JAM-A (F11R or CD321) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-JAM-A (F11R or CD321) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-JAM-A (F11R or CD321) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Junctional adhesion molecule A (JAM-A) is a protein that is encoded by the F11R gene in human. JAM-A is also known as CD321.

JAM proteins feature two extracellular immunoglobulin domains, a single transmembrane domain, and a cytoplasmic domain with a PDZ-binding motif. JAM-A can interact with ZO-1 via its PDZ-binding motif.

JAM-A is expressed in a variety of epithelial tissues.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (14-aa) derived from the C-terminal region of human JAM-A protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Tight Junction Protein

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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JAM-A (F11R or CD321) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Tight Junction Protein

Reviews

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