JAM-B (JAM2) antibody labeling of mouse tissue

JAM-B (JAM2 or CD322) antibody

Name: JAM-B (JAM2 or CD322) antibody
SKU: 00252
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00252

Antibody: Rabbit JAM-B (JAM2) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-JAM-B (JAM2) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

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Optional Blocking Peptide

No blocker

1 ug blocker + $69.00

10 ug blocker + $119.00

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Anti-JAM-B (JAM2) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Junctional adhesion molecule B (JAM-B) is a protein that is encoded by the JAM-2 gene in human. JAM-B is also known as CD322.

JAM proteins feature two extracellular immunoglobulin domains, a single transmembrane domain, and a cytoplasmic domain with a PDZ-binding motif. JAM-B can interact with the polarity protein Par3 and the focal adhesion protein – integrins.

JAM-B is expressed by the endothelium and sometimes referred to as vasculoendothelial JAM (VE-JAM).

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal region of human JAM-B protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Tight Junction Protein

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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JAM-B (JAM2 or CD322) antibody

Optional Blocking Peptide

Protocol for Staining Tight Junction Protein

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