JAM-B (JAM2) antibody labeling of mouse tissue

JAM-B (JAM2) antibody labeling of the developing heart

JAM-B proteins are found in the blood vessels of the heart from an embryonic mouse at E15.5.

JAM-B in the developing intestine

JAM-B proteins are found in the blood vessels of the lamina propria (LP) in the small intestine from an embryonic mouse at E15.5. Note that the tight junctions in the mucosal epithelium express ZO-1 but not JAM-B.

JAM-B (JAM2 or CD322) antibody

Name: JAM-B (JAM2 or CD322) antibody
SKU: 00252
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00252

Antibody: Rabbit JAM-B (JAM2) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-JAM-B (JAM2) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-JAM-B (JAM2) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Junctional adhesion molecule B (JAM-B) is a protein that is encoded by the JAM-2 gene in human. JAM-B is also known as CD322.

JAM proteins feature two extracellular immunoglobulin domains, a single transmembrane domain, and a cytoplasmic domain with a PDZ-binding motif. JAM-B can interact with the polarity protein Par3 and the focal adhesion protein – integrins.

JAM-B is expressed by the endothelium and sometimes referred to as vasculoendothelial JAM (VE-JAM).

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal region of human JAM-B protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Tight Junction Protein

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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JAM-B (JAM2 or CD322) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Tight Junction Protein

Reviews

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