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KCND3 (Kv4.3) antibody labeling of mouse cerebellum

KCND3 (Kv4.3) antibody labeling of mouse cerebellum

Kv4.3 proteins are expressed by the granular neurons in the mouse cerebellum. Mol., molecular layer; Gr., granular layer.

KCND3 (Kv4.3) antibody

$155.00 – $435.00

Item Cat No.: 20243

Antibody: Rabbit KCND3 (Kv4.3) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-KCND3 (Kv4.3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

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Anti-KCND3 (Kv4.3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Potassium voltage-gated channel subfamily D member 3 (KCND3), also known as Kv4.3, is is a shal-related voltage-gated potassium channel that is encoded by the KCND3 gene in human. Kv4.3 plays important roles in the repolarization phase of the action potential. Gain of function in the KCND3 gene is believed to cause Brugada syndrome.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (13-aa) derived from the C-terminal region of mouse Kv4.3 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 92.3% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

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Univ.-Prof. Jay Gopalakrishnan PhD

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"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!

Annette Ehrhardt, PhD

Dept. of Pediatrics | Emory University

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