LGR5 (GPR49 or GPR67) antibody labeling of mouse colon

LGR5 proteins decorate the intestinal stem cells in the mouse colon. Note the lineage development from an intestinal stem cell (arrow).

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LGR5 (GPR49 or GPR67) antibody

Name: LGR5 (GPR49 or GPR67) antibody
SKU: 50630
Availability: InStock

$155.00 – $435.00

Item Cat No.: 50630

Antibody: Rabbit LGR5 (GPR49 or GPR67) polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-LGR5 (GPR49 or GPR67) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-LGR5 (GPR49 or GPR67) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67), is encoded by the LGR5 gene in human. LGR5 is a member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. LGR5 is a receptor for R-spondin and is involved in the canonical Wnt signaling pathway. LGR5 plays a role in the maintenance of adult intestinal stem cells during post-embryonic development.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (12-aa) derived from the C-terminal region of human LGR5 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining G-Protein Coupled Receptor

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]

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LGR5 (GPR49 or GPR67) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining G-Protein Coupled Receptor

Reviews

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