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Maleimide-Activated Carrier Protein (KLH or BSA or OVA)

$125.00$505.00

Item Cat No.: BCADMA

Concentration: 10 mg/ml of carrier protein

Application: Peptide Conjugation

Reactivity: Rabbit, Rat, Mouse

BiCell Scientific Inc has developed a series of preactivated carrier proteins allowing rapid conjugation of peptides to induce immune responses.

Carrier proteins are large, complex molecules capable of stimulating an immune response upon injection. Successful production of antibodies specific to small hapten antigens (i.e., peptides or drug compounds) requires that these haptens be covalently conjugated to a larger, more complex carrier protein to make them immunogenic.

BiCell Scientific’s maleimide-activated carrier proteins enable conjugation of sulfhydryl-containing peptide haptens to the highly immunogenic keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA) or ovalbumin (OVA) carrier protein for immunization and antibody production. These carrier proteins are rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated proteins are then stabilized in PBS based buffers. Maleimide groups can then form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens.

BiCell Scientific’s maleimide-activated KLH protein contains 400 moles of sulhydryl-reactive maleimide group per mole of KLH; activated BSA protein contains 20 moles of maleimide group per mole of BSA; activated OVA protein contains 15 moles of maleimide group per mole of OVA. Due to the large number of available conjugation sites, KLH is the primary choice of inducer of immunogenic reaction. BSA or OVA is often used as an irrelevant protein carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against a hapten.

Each kit contains columns and reagents sufficient for 1-5 independent conjugation reactions.

  • Preactivated carrier protein (0.2 ml to 1 ml)

Store at -20oC.

  • 1-5 desalting columns (bed volume: 1 ml)

Store at 4oC.


For Research Use Only. Not for use in clinical diagnostics.

Step 1. Conjugation reaction

  • Dissolve 2mg of sulfhydryl-containing hapten (e.g. peptide) in a volume of 200μl of 1x PBS;
  • Add 20oμl of maleimide-activated carrier protein (2mg) to the hapten solution;
  • Rotate at room temperature for 1hr.

Step 2. Purification of conjugated protein

  • Centrifuge desalting column at 1,000 xg for 5min;
  • Add 1ml of 1x PBS and centrifuge at 1,000 xg for 5min, and repeat for 3 times;
  • Add the conjugation reaction solution (400μl) and centrifuge at 1,000 xg for 5min;
  • Collect elutant.

Additional information

Carrier protein

KLH, BSA, OVA

Scale

2 mg, 5x 2 mg

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Be the first to review “Maleimide-Activated Carrier Protein (KLH or BSA or OVA)”

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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