MYLK (MLCK or MLCK1) HiRe rabbit antibody

Anti-MYLK (MLCK or MLCK1) HiRe (High-Resolution) rabbit antibody is validated on human tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from human and rodent tissues.

Myosin light chain kinase (MYLK or MLCK) is a calcium/calmodulin dependent enzyme that is encoded by the MYLK gene in human. MYLK phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. The MYLK gene encodes both smooth muscle and nonmuscle isoforms.

In smooth muscle, the 130-kDa smooth muscle MYLK/MLCK (short MLCK) and a carboxyl-terminal fragment of MLCK, telokin, are produced as alternatively spliced mRNA transcripts. In contrast, a 220-kDa MYLK/MLCK (long MLCK or MLCK1) is expressed in many nonmuscle cells, including epithelium and endothelium.

This antibody was raised against an epitope that only exists in the full-length long MLCK or MLCK1. The epitope – VSGIPKPEVAWFLEG is an improved version and offers better signal resolution than the previous epitope described and published before: KARTRDSGTYSCTASNAQ (Clayburgh and colleagues, 2004).