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MYLK (MLCK or MLCK1) HiRe rabbit antibody


Item Cat No.: 00257

Antibody: Rabbit MYLK (MLCK or MLCK1) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-MYLK (MLCK or MLCK1) HiRe (High-Resolution) rabbit antibody is validated on human tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from human and rodent tissues.

Optional Blocking Peptide

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Anti-MYLK (MLCK or MLCK1) HiRe (High-Resolution) rabbit antibody is validated on human tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from human and rodent tissues.

Myosin light chain kinase (MYLK or MLCK) is a calcium/calmodulin dependent enzyme that is encoded by the MYLK gene in human. MYLK phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. The MYLK gene encodes both smooth muscle and nonmuscle isoforms.

In smooth muscle, the 130-kDa smooth muscle MYLK/MLCK (short MLCK) and a carboxyl-terminal fragment of MLCK, telokin, are produced as alternatively spliced mRNA transcripts. In contrast, a 220-kDa MYLK/MLCK (long MLCK or MLCK1) is expressed in many nonmuscle cells, including epithelium and endothelium.

This antibody was raised against an epitope that only exists in the full-length long MLCK or MLCK1. The epitope – VSGIPKPEVAWFLEG is an improved version and offers better signal resolution than the previous epitope described and published before: KARTRDSGTYSCTASNAQ (Clayburgh and colleagues, 2004).

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (15-aa) derived from the internal region of human MYLK protein

Species homology of immunogen: Synthetic peptide sequence shows 86.7% homology to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!

Annette Ehrhardt, PhD

Dept. of Pediatrics | Emory University

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