NEPH2 (KIRREL3) antibody labeling of mouse kidney

NEPH2 proteins are expressed exclusively by the podocytes in the glomerulus of the mouse kidney.

NEPH2 (KIRREL3) antibody

Name: NEPH2 (KIRREL3) antibody
SKU: 00178
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00178

Antibody: Rabbit NEPH2 (KIRREL3) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-NEPH2 (KIRREL3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-NEPH2 (KIRREL3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

NEPH2, also known as Kin of IRRE-like protein 3 (KIRREL3), is encoded by the KIRREL3 gene in human. NEPH2 is a member of the NEPH protein family, which includes NEPH1 (KIRREL1) and NEPH3 (KIRREL2).

NEPH2 is found in the slit diaphragm of the kidney podocytes and can interact with Nephrin and CASK. NEPH2 consists of an extracellular N-terminal domain, a helical transmembrane domain, and a cytoplasmic C-terminal domain. NEPH2 has also been implicated in synapse formation. Genetic knockout of NEPH2 cause abnormal brain development.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (18-aa) derived from the C-terminal region of human NEPH2 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Slit Diaphragm Protein

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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NEPH2 (KIRREL3) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Slit Diaphragm Protein

Reviews

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