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Nephrocystin-2 (NPHP2 or Inversin or INVS) antibody labeling of mouse tissue

Nephrocystin-2 (NPHP2 or Inversin or INVS) antibody labeling of the mesothelium

Nephrocystin-2 proteins are found in the primary cilium of the mesothelium surrounding the colon in an embryonic mouse at E15.5. muc., mucosa; m.m., muscularis mucosae; mes., mesothelium.

NPHP2 in the myocardium

Nephrocystin-2 proteins are found in the centrosomes or basal bodies of the myocardium from an embryonic mouse at E15.5.

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Nephrocystin-2 (NPHP2 or Inversin or INVS) antibody

$155.00 – $435.00

Item Cat No.: 90002

Antibody: Rabbit Nephrocystin-2 (NPHP2 or Inversin or INVS) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Mouse, Rat

Anti-Nephrocystin-2 (NPHP2 or Inversin or INVS) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

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Anti-Nephrocystin-2 (NPHP2 or Inversin or INVS) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Nephrocystin-2 (NPHP2) is also known as Inversin. It is encoded by the INVS gene in human. Nephrocystin-2 contains multiple ankyrin domains and two IQ calmodulin-binding domains. It is a component of the primary cilium and plays a vital role in left-right axis determination.

Mutations in Nephrocystin-2 cause nephronophthisis type 2 (OMIM 602088), which manifests as renal failure and acidosis, accompanied by hypertension and polyuria.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (17-aa) derived from the C-terminal region of mouse Nephrocystin-2 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 58.8% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Primary Cilium (embryo section) (methanol fixation)

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Protocol for Staining Primary Cilium (retina section)

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 […]

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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