Nephrocystin-6 (NPHP6 or CEP290 or MKS4 or BBS14) antibody labeling of mouse retina

CEP290 proteins are found in the basal body/centriole of the connecting cilia (primary cilia) of the photoreceptor cells and the centrosomes of the bipolar cells in the mouse retina. IS: inner segment; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer.

NPHP6 (CEP290) in P10 mouse retina

Courtesy of Dr. Abigail Moye (Baylor College of Medicine)

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Nephrocystin-6 (NPHP6 or CEP290 or MKS4 or BBS14) antibody

Name: Nephrocystin-6 (NPHP6 or CEP290 or MKS4 or BBS14) antibody
SKU: 90006
Availability: InStock

$155.00 – $435.00

Item Cat No.: 90006

Antibody: Rabbit Nephrocystin-6 (NPHP6 or CEP290 or MKS4 or BBS14) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Mouse, rat

Anti-Nephrocystin-6 (NPHP6 or CEP290 or MKS4 or BBS14) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-Nephrocystin-6 (NPHP6 or CEP290 or MKS4 or BBS14) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent tissues.

Nephrocystin-6, also known as centrosomal protein 290 (CEP290), or MKS3 or BBS14, is encoded by the CEP290 gene in human. The CEP290 protein is a centrosomal protein that plays an important role in the development of centrosome and primary cilium. It contains several coiled-coil domains and an ATP/GTP binding motif.

Mutations in CEP290 has been associated with nephronophthisis, Meckel syndrome, and Joubert syndrome (OMIM 610142).

This antibody is recommended for staining the centrosomes in rodent tissues. For human tissues, please choose 90006h.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (15-aa) derived from the N-terminal region of mouse CEP290 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 86.7% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Primary Cilium (retina section)

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 […]

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Nephrocystin-6 (NPHP6 or CEP290 or MKS4 or BBS14) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Primary Cilium (retina section)

Reviews

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