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NHE3 (Slc9a3) antibody labeling of mouse tissue

NHE3 (Slc9a3) antibody labeling of the kidney

NHE3 proteins are localized to the luminal membrane of the epithelium making the proximal tubule (counterstained by Lotus tetragonolobus lectin (LTL)) in the mouse kidney.

NHE3 in the colon

NHE3 proteins are localized to the luminal membrane of the epithelium in the mucosal layer of the mouse colon.

NHE3 (Slc9a3) antibody

$155.00 – $435.00

Item Cat No.: 21003

Antibody: Rabbit Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-NHE3 (Slc9a3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

Product price:$155.00
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Anti-NHE3 (Slc9a3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Sodium-hydrogen exchanger 3 (NHE3), or sodium-hydrogen antiporter 3, is encoded by the SLC9A3 gene in human. NHE3 is an epithelial brush border Na/H exchanger that uses an inward sodium ion gradient to expel acids from the cell.

NHE3 consists in 10 transmembrane domains. NHE3 is found on the luminal membrane of the epithelial cells of the proximal tubule in the kidney and the epithelial cells in the intestinal mucosa.

Mutations in NHE3 are a cause of congenital secretory sodium diarrhea (OMIM 616868).

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse NHE3 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 62.5% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!

Annette Ehrhardt, PhD

Dept. of Pediatrics | Emory University

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