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NKCC1 (Slc12a2) antibody labeling of mouse tissue

NKCC1 (Slc12a2) antibody labeling of the choroid plexus

NKCC1 proteins are found in the luminal membrane of the epithelium lining the choroid plexus (c.p.) in the mouse brain. The endothelium in the choroid plexus is labeled with Calbindin D28K.

NKCC1 (Slc12a2) antibody

$155.00 – $435.00

Item Cat No.: 20302

Antibody: Rabbit NKCC1 (Slc12a2) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Mouse, rat

Anti-NKCC1 (Slc12a2) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

Product price:$155.00
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Anti-NKCC1 (Slc12a2) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent tissues.

The Na-K-Cl cotransporter type 1 (NKCC1) protein is encoded by the SLC12A2 gene in human. NKCC1 consists in 12 transmembrane domains and the stoichiometry of the transported solutes is 1Na:1K:2Cl.

NKCC1 is highly expressed in organs that secrete fluids, such as exocrine glands and choroid plexus. In addition to exocrine glands, NKCC1 is important for establishing the potassium-rich endolymph that bathes the cochlea in the inner ear.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the N-terminal cytoplasmic region of mouse NKCC1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 43.8% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!

Annette Ehrhardt, PhD

Dept. of Pediatrics | Emory University

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