P-Cadherin (Cadherin-3 or CDH3) antibody

$155.00 – $435.00

Item Cat No.: 00103

Antibody: Rat P-Cadherin (Cadherin-3 or CDH3) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-P-Cadherin (Cadherin-3 or CDH3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Cadherin-3, also known as P-Cadherin, is a cadherin protein that is encoded by the CDH3 gene in human. Cadherin-3 is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail.

Cadherin-3 was first described as a cadherin molecule most abundant in the developing mouse placenta – hence “P-cadherin”.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (17-aa) derived from the C-terminal intracellular region of mouse P-cadherin protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 88.2% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Cadherin

Name: P-Cadherin (Cadherin-3 or CDH3) antibody
SKU: 00103
Availability: InStock

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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P-Cadherin (Cadherin-3 or CDH3) antibody

Optional Blocking Peptide

Protocol for Staining Cadherin

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