Par3 (PARD3) antibody labeling of mouse kidney

Par3 proteins are localized to the tight junctions of the tubular epithelia in the mouse kidney.

Par3 in the liver

Par3 proteins are localized to the tight junctions in the bile canaliculi from the mouse liver.

Par3 (PARD3) antibody

Name: Par3 (PARD3) antibody
SKU: 00248
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00248

Antibody: Rabbit Par3 (PARD3) polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, Mouse, Rat

Anti-Par3 (PARD3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-Par3 (PARD3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Partitioning defective 3 homolog (PARD3), also known as Par3, is encoded by the PARD3 gene in human. Par proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized cell growth.

Par3 is a membrane adapter protein containing multiple PDZ domains. Par3 is important for establishing not only apicobasal polarity, but also apical junctions.

Par3 is expressed by a variety of epithelial cell types.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (17-aa) derived from the C-terminal region of human Par3 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Tight Junction Protein

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Par3 (PARD3) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Tight Junction Protein

Reviews

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