Par6b (PARD6B) antibody labeling of mouse tissue

Par6b (PARD6B) antibody labeling of the developing intestine

Par6b proteins are found in the luminal membrane of the small intestine in a mouse embryo at E15.5.

Par6b in the developing lung

Par6b proteins are expressed in the apical membrane of the branching bronchi in a mouse embryo at E15.5.

Par6b in the developing kidney

Par6b proteins are localized to the apical membrane of the kidney tubules from a mouse embryo at E15.5.

Par6b (PARD6B) antibody

Name: Par6b (PARD6B) antibody
SKU: 00250
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00250

Antibody: Rabbit Par6b (PARD6B) polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, Mouse, Rat

Anti-Par6b (PARD6B) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-Par6b (PARD6B) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Partitioning defective 6 homolog beta (PARD6B), also known as Par6b, is encoded by the PARD6B gene in human. Par proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized cell growth.

Par6b contains a PSD95/Discs-large/ZO1 (PDZ) domain and a Cdc42/Rac interactive binding (CRIB) domain.

Par6b is a cytoplasmic protein involved in asymmetrical cell division and cell polarization processes.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of human Par6b protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Tight Junction Protein

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Par6b (PARD6B) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Tight Junction Protein

Reviews

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