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Pendrin (Slc26a4) antibody labeling of mouse kidney

Pendrin (Slc26a4) antibody labeling of mouse kidney

Pendrin proteins are found in the luminal membrane of the intercalated cells of the collecting duct in the mouse kidney. Note that aquaporin-2 (aqp2) proteins are in the principal cells of the collecting duct.

Dual-color fluorescent IHC on FFPE section

FFPE section of mouse kidney was immunostained by anti-Calbindin and anti-Pendrin antibodies simultaneously and imaged by two fluorophores. Note that Calbindin antibody labels the cytoplasm of the principal cells in the DCT2 tubules, while Pendrin antibody labels the luminal membrane of the intercalated cells in the DCT2 and collecting duct tubules.

Delineating cell lineage in the collecting duct on FFPE section

Anti-Aqp2 antibody labels the principal cells while anti-Pendrin antibody labels the beta-intercalated cells in the collecting duct of the mouse kidney.

Differentiating alpha from beta intercalated cell in the collecting duct on FFPE section

Anti-Pendrin antibody labels the luminal surface of beta-intercalated cells while anti-ATP6B1 antibody labels the luminal membrane of alpha-intercalated cells, as well as the luminal and basolateral membrane of beta-intercalated cells.

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Pendrin (Slc26a4) antibody

$155.00 – $435.00

Item Cat No.: 20501

Antibody: Rabbit Pendrin (Slc26a4) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-Pendrin (Slc26a4) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

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Anti-Pendrin (Slc26a4) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Pendrin is an anion exchanger that is encoded by the Slc26a4 gene in human. Pendrin mediates electroneutral exchange of chloride for bicarbonate across the plasma membrane of epithelial cells.

Pendrin is a membrane protein with 12 transmembrane domains and molecular weight of 86 kDa.

Mutations in Pendrin cause Pendred syndrome or DFNB4 (OMIM 274600), an autosomal recessive disorder characterized by sensorineural deafness and thyroid goiter. Pendrin is highly expressed by the kidney and the inner ear.

This antibody can be used with anti-Aqp2 or anti-Atp6v1b1 to differentiate the cell types in kidney collecting ducts.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of mouse pendrin protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 93.3% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Delineating cell lineage in the collecting duct of mouse kidney

Protein Atlas in Cell and Tissue

Differentiating principal cell from intercalated cell Expand The collecting duct in the kidney consists in three types of cells, principal cells, alpha-intercalated cells and beta-intercalated cells. The principal cells are primarily responsible for salt and water transport while alpha- and beta-intercalated cells are responsible for acid-base transport. Figure legend Anti-Aqp2 antibody labels the luminal membrane […]

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Dual-color fluorescent IHC on FFPE section

Fixation Techniques and Artifacts

Low-magnification fluorescent imaging Expand FFPE sections were obtained from formalin (10% neutral formaldehyde) fixed mouse kidney tissues. Antigens on FFPE sections were retrieved by citrate solution at pH 6.0. Two BiCell Scientific antibodies, raised in rat and rabbit respectively, were incubated with FFPE sections to detect two independent antigen proteins. Figure legend Fluorescent IHC on […]

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Protocol for Staining Pendrin

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

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Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

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