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Pericentrin-1 (Nup85) antibody

$99.00$295.00

Item Cat No.: 01063

Antibody: Rat Pericentrin-1 (Nup85) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

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Anti-Pericentrin-1 (Nup85) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Pericentrin-1, also known as Nucleoporin 85 (Nup85), is encoded by the NUP85 gene in human. Nup85 is an essential component of the nuclear pore complex (NPC) and required for NPC assembly and maintenance. Nup85 is also a highly conserved centrosomal protein involved in microtubule organization.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (18-aa) derived from the C-terminal region of human Pericentrin-1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Centrosome (embryo section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Blocking buffer Expand NaCl 8 g KCl 0.2 g […]

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Protocol for Staining Centrosome (retina section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 g […]

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