PRICKLE1 (RILP) antibody labeling of embryonic mouse brain

PRICKLE1 proteins are expressed by the developing neurons in the forebrain of a mouse embryo at E17.5.

PRICKLE1 in embryonic mouse hindbrain

PRICKLE1 proteins are expressed by the developing neurons in the hindbrain of a mouse embryo at E17.5.

PRICKLE1 (RILP) antibody labeling of mouse retina

PRICKLE1 proteins are found in the cytoplasm of the photoreceptors in the ONL and the neurons in the INL and GCL of the mouse retina. ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer.

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PRICKLE1 (RILP) antibody

Name: PRICKLE1 (RILP) antibody
SKU: 50621
Availability: InStock

$155.00 – $435.00

Item Cat No.: 50621

Antibody: Rabbit PRICKLE1 (RILP) polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-PRICKLE1 (RILP) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-PRICKLE1 (RILP) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Prickle planar cell polarity protein 1 (PRICKLE1), also known as RILP, is encoded by the PRICKLE1 gene in human. PRICKLE1 is a nuclear receptor that acts as a negative regulator of the Wnt/beta-catenin signaling pathway. PRICKLE1 is also involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (15-aa) derived from the N-terminal region of mouse PRICKLE1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 93.3% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining PRICKLE1-2 (retina section)

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBSNaCl 8 gKCl 0.2 gNa2HPO4 1.44 […]

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PRICKLE1 (RILP) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining PRICKLE1-2 (retina section)

Reviews

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