Protocadherin-15 (PCDH15) antibody labeling of embryonic mouse ganglion

Protocadherin-15 proteins are found in the primordial neurons of the dorsal root ganglion from a mouse embryo at E15.5.

Protocadherin-15 in embryonic mouse spinal nerve

Protocadherin-15 proteins are found in the nerve fibers of the marginal layer of spinal cord as well as the neighboring ganglion of a mouse embryo at E15.5.

Protocadherin-15 in embryonic mouse skin

Protocadherin-15 proteins are expressed in the epithelium of the epidermis of the skin from a mouse embryo at E15.5.

Protocadherin-15 in mouse embryo

Panoramic view of Protocadherin-15 in mouse embryo section (E15.5). (BiCell Blue Image)

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Protocadherin-15 (PCDH15 or CDHR15 or USH1F or DFNB23) antibody

Name: Protocadherin-15 (PCDH15 or CDHR15 or USH1F or DFNB23) antibody
SKU: 50535
Availability: InStock

$155.00 – $435.00

See High Resolution Histopathology Image

Item Cat No.: 50535

Antibody: Rat Protocadherin-15 (PCDH15 or CDHR15 or USH1F or DFNB23) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Mouse, rat

Anti-Protocadherin-15 (PCDH15 or CDHR15 or USH1F or DFNB23) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Protocadherin-15 is a cadherin protein encoded by the PCDH15 gene in human. Protocadherin-15 belongs to the protocadherin subfamily, which is a membrane protein found at cell-cell boundaries. Protocadherin-15 plays an essential role in the maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss (DFNB23) and Usher Syndrome Type IF (USH1F).

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (13-aa) derived from the C-terminal region of mouse protocadherin-15 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 69.2% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Protocadherin (embryo section)

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Protocadherin-15 (PCDH15 or CDHR15 or USH1F or DFNB23) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Protocadherin (embryo section)

Reviews

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