Protocadherin-18 (PCDH18 or PCDH68L) antibody labeling of embryonic mouse skin

Protocadherin-18 proteins are found in the cell junctions of the epithelial cells in the epidermis of the skin from a mouse embryo at E15.5.

Protocadherin-18 (PCDH18 or PCDH68L) antibody

Name: Protocadherin-18 (PCDH18 or PCDH68L) antibody
SKU: 50538
Availability: InStock

$155.00 – $435.00

Item Cat No.: 50538

Antibody: Rat Protocadherin-18 (PCDH18 or PCDH68L) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-Protocadherin-18 (PCDH18 or PCDH68L) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-Protocadherin-18 (PCDH18 or PCDH68L) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Protocadherin-18 is a cadherin protein encoded by the PCDH18 gene in human. Protocadherin-18 belongs to the protocadherin subfamily, which is a membrane protein found at cell-cell boundaries. Protocadherin-18 protein includes an extracelllular domain, containing 6 cadherin-like motifs, a transmembrane domain and a C-terminal cytoplasmic domain.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal region of human protocadherin-18 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Protocadherin (embryo section)

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Protocadherin-18 (PCDH18 or PCDH68L) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Protocadherin (embryo section)

Reviews

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