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Recombinant Flag Monoclonal Antibody

$205.00$1,250.00

Item Cat No.: BCRA03

Antibody: Monoclonal Antibody

Concentration: 0.50 mg/ml

Application: In vitro and In vivo

Reactivity: Human, Mouse, Rat

Recombinant Flag monoclonal antibody is a chimeric anti-Flag tag antibody consisting of the Fv domain sequence of mouse hybridoma clone M2 and the Fc domain sequence of either mouse, rat or rabbit.

Flag tag is a short peptide sequence (DYKDDDDK) commonly used as a fusion partner to facilitate protein purification experiments. BiCell Scientific Inc’s anti-Flag antibody utilizes the Fv domain sequence of mouse hybridoma clone M2 that is the best characterized anti-Flag clone.

BiCell Scientific Inc’s anti-Flag antibody is recombinant chimeric antibody that combines the Fv domain of M2 with the Fc domain of a selected species from mouse, rat or rabbit. Such a feature allows the chimeric antibody to retain the original binding characteristics of M2 clone while confer a greater species flexibility.

Isotype: IgG1

Class: Recombinant monoclonal

Immunogen: DYKDDDDK

Production platform: CHO cells

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics or therapeutics.

Additional information

Antibody Production Scale

50 ug, 250 ug, 1 mg

Species Determinant Fc Domain

Mouse, Rat, Rabbit

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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