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ROBO2 antibody labeling of embryonic mouse brain

ROBO2 antibody labeling of embryonic mouse brain

ROBO2 proteins are found in neurons of the striatum from the forebrain of a mouse embryo at E15.5.

ROBO2 antibody

$155.00 – $435.00

Item Cat No.: 50832

Antibody: Rat ROBO2 polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-ROBO2 antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

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Anti-ROBO2 antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Roundabout homolog 2 is a membrane protein that is encoded by the ROBO2 gene in human. Human ROBO2 protein is structurally similar to a Drosophila integral membrane protein that is encoded by the Drosophila roundabout gene (a member of the immunoglobulin gene superfamily). ROBO2 is a receptor protein for the slit homolog 2 protein and functions in axon guidance and cell migration.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse ROBO2 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 81.3% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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