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ROBO4 antibody labeling of embryonic mouse heart

ROBO4 antibody labeling of embryonic mouse heart

ROBO4 proteins are found in the neuromuscular junctions of the cardiac muscle cells in the heart of a mouse embryo at E15.5.

ROBO4 in embryonic mouse intercostal muscle

ROBO4 proteins are found in the neuromuscular junctions of the intercostal muscle cells of a mouse embryo at E15.5.

ROBO4 antibody

$155.00 – $435.00

Item Cat No.: 50834

Antibody: Rat ROBO4 polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-ROBO4 antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

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Anti-ROBO4 antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Roundabout homolog 4 is a membrane protein that is encoded by the ROBO4 gene in human. Human ROBO4 protein is structurally similar to a Drosophila integral membrane protein that is encoded by the Drosophila roundabout gene (a member of the immunoglobulin gene superfamily). ROBO4 protein plays a role in angiogenesis and establishment of the endothelial barrier. ROBO4 protein is implicated in aortic valve disease 3.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse ROBO4 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 87.5% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!

Annette Ehrhardt, PhD

Dept. of Pediatrics | Emory University

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