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Sarcoglycan Epsilon (SGCE) antibody labeling of embryonic mouse cardiac muscle cells

Sarcoglycan Epsilon (SGCE) antibody labeling of embryonic mouse cardiac muscle cells

SGCE proteins are found in the plasma membrane of the primordial cardiac muscle cells in the heart of a mouse embryo at E15.5.

Sarcoglycan Epsilon (SGCE) antibody

$155.00 – $435.00

Item Cat No.: 54704

Antibody: Rat Sarcoglycan Epsilon (SGCE) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-Sarcoglycan Epsilon (SGCE) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

Product price:$155.00
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Anti-Sarcoglycan Epsilon (SGCE) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Epsilon-sarcoglycan is a membrane protein that is encoded by the SGCE gene in human. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Unlike other family members which are predominantly expressed in striated muscle, the epsilon sarcoglycan is more broadly expressed. Mutations in this gene are associated with myoclonus-dystonia syndrome.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse Sarcoglycan Epsilon protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 93.8% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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Univ.-Prof. Jay Gopalakrishnan PhD

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"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!

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