Synaptopodin (SYNPO) antibody labeling of mouse kidney

Synaptopodin proteins are found in the glomerulus of the mouse kidney.

Synaptopodin in the glomerular podocytes

Synaptopodin proteins are found in the cytoskeleton of the podocytes in the kidney glomerulus.

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Synaptopodin (SYNPO) antibody

Name: Synaptopodin (SYNPO) antibody
SKU: 00175
Availability: InStock

$155.00 – $435.00

Item Cat No.: 00175

Antibody: Rabbit Synaptopodin (SYNPO) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-Synaptopodin (SYNPO) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$155.00
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Anti-Synaptopodin (SYNPO) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Synaptopodin is encoded by the SYNPO gene in human. Synaptopodin is an actin-associated protein that plays a role in actin-based cell shape and motility. Synaptopodin proteins are found in postsynaptic densities and dendritic spines of the neurons and in glomerular podocytes of the kidney.

Synaptopodin has been shown to interact with CD2AP and Actinin alpha-4 in the slit diaphragm made by the glomerular podocytes. Mutations in SYNPO gene have been associated with focal segmental glomerulosclerosis in man.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (18-aa) derived from the C-terminal region of human Synaptopodin protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Slit Diaphragm Protein

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Synaptopodin (SYNPO) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Slit Diaphragm Protein

Reviews

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