Name: Antibody Sequencing &amp; Cloning Service
SKU: BCABSE
Price: 3150.00 USD
Availability: InStock

Antibody Sequencing & Cloning Service

$3,150.00

Item Cat No.: BCABSE

Antibody: Monoclonal Antibody

Application: Sequencing and cloning

Reactivity: Human, rabbit, rodent

BiCell Scientific’s antibody sequencing & cloning service provides full-length sequencing of custom antibody from hybridoma cell line and reassembly into mammalian antibody expression vector.

Determining an antibody’s sequence is crucial for various downstream applications, including antibody engineering, affinity maturation, functional optimization, and database documentation.

BiCell Scientific’s antibody sequencing & cloning service provides full-length sequencing of custom antibody from hybridoma cell line using 5′-RACE PCR technique. The fully sequenced antibody will then be cloned into a mammalian expression vector for recombinant antibody production.

Steps	Timeline	Deliverables	QC standard
Phase I: Total RNA extraction from hybridoma cell pellet (Total RNA will be extracted and purified from hybridoma cell pellet.)	1 week	/	COA
Phase II: Reverse transcription (Total RNA will be transcribed into cDNA using either an Oligo(dT) or a C_H/C_L antisense primer.)	1-2 weeks	/	COA
Phase III: 5′ RACE amplification of heavy and light chains (For 5’RACE PCR, heavy and light chains will be amplified with our proprietary adaptor (sense primer) and a gene specific (C_H/C_L, reverse primer). PCR products will include the sequence of the signal peptide, variable domains and constant domains up to the reverse primer.)	2-3 weeks	/	COA
Phase IV: Cloning into sequencing vector (PCR products will be gel purified and cloned into a sequencing vector for sequencing.)	1-2 weeks	Detailed report including the sequence alignments of the heavy and light chains sent to customer	Sequencing

Customers will receive the following deliverables at the end of each project:

Fully sequenced F_v domains of heavy chain and light chain of customer’s antibody
Cloning F_v domains of heavy chain and light chain into pFUSE series of mammalian F_c expression vectors

For Research Use Only. Not for use in clinical diagnostics or therapeutics.

Total RNA extraction from the hybridoma cell pellet – 1-2 weeks
Reverse transcription – 1-2 weeks
5′ RACE amplification of heavy and light chains – 2-3 weeks
Cloning into sequencing vector – 1-2 weeks
Sequencing 5 clones – 1-2 weeks
Cloning F_v domain into pFUSE series of F_c domain – 1-2 weeks

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Antibody Sequencing & Cloning Service

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