Protocol for Staining Primary Cilia (cell culture)
General and Specific ProtocolsMaterials and reagents Expand Equipment Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Blocking buffer Expand BLOCK Solution NaCl 8 g KCl 0.2 g Na2HPO4 1.44 g KH2PO4 0.24 g Tween-20 2 ml Fetal bovine serum 50 ml […]