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Educational Articles

Protocol for Staining Pendrin

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]

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Practical consideration of fixation technique

Concentration of fixative Expand Experiments have been performed to reveal the effects of different concentrations of aldehyde fixatives on tissue morphology. Over a 10-fold range, varying the concentrations of formaldehyde from 2% to 20% in a fixative solution had little effect on the cytoplasmic volume in one-centimeter blocks of rat kidney biopsies. Only at very […]

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Protocol for Staining CLDN16 (phosphorylated) Protein

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]

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Protocol for Staining LAMB2 Protein

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 2% paraformaldehyde in PBS NaCl 8 […]

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Protocol for Staining ACTN4 Protein

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 2% paraformaldehyde in PBS NaCl 8 […]

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Protocol for Staining CD2AP Protein

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 2% paraformaldehyde in PBS NaCl 8 […]

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Protocol for Staining Centrosome (embryo section)

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Blocking buffer Expand BLOCK Solution  NaCl 8 g […]

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Protocol for Staining Centrosome (retina section)

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 […]

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Protocol for Staining Laminin (unfixed tissue)

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution  NaCl 8 g KCl […]

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