Matrigel coating of culture dish Expand 1. Thaw Ready-to-use Matrigel on ice. Coat dish (35-mm to 150-mm diameter) by adding 500μl – 5ml ready-to-use gelatin solution to cover the bottom; rock the dish back and forth to let solution cover homogeneously. 2. Sit at room temperature for 1hr (keep sterile in culture hood). 3. Aspirate solution and […]
Gelatin coating of culture dish Expand 1. Coat dish (35-mm to 150-mm diameter) by adding 500μl – 5ml ready-to-use gelatin solution to cover the bottom; rock the dish back and forth to let solution cover homogeneously; sit at room temperature for 3hr (keep sterile in culture hood). 2. Aspirate solution; allow air dry overnight in culture hood […]
RNAi oligo design program Expand RNA interference oligo design algorithm is written into an online program “RNAi Score Calculator”. Clicking the program will enter the interface for inputting target gene cDNA sequence. Input target gene cDNA sequence Expand After entering “RNAi Score Calculator” interface, target gene cDNA sequence (FASTA format) can be pasted into the […]
Materials and reagents Expand Equipment Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Blocking buffer Expand BLOCK Solution NaCl 8 g KCl 0.2 g Na2HPO4 1.44 g KH2PO4 0.24 g Tween-20 2 ml Fetal bovine serum 50 ml […]
Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]
Preparation of Myeloma Cells Expand Prewarm all culture media to 37oC. Thaw the parental myeloma cells (Sp2/0-Ag14 or X63-Ag8.653) from liquid nitrogen and culture in BiCell-mAb Medium MY for 3-5 days to fully recover cell growth to reach 1 x 106/ml cell density. Split cells at 1:5 into fresh BiCell-mAb Medium MY and let grow […]
Coating antigen to microplate Expand 1, Dilute the antigen to a final concentration of 1 µg/ml (peptide antigen) or 10 µg/ml (protein antigen) in PBS. Coat the wells of a PVC 96-well plate with the antigen by pipetting 100 µl of the antigen dilution. 2, Cover the plate with an adhesive plastic and incubate at […]
Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]
Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution NaCl 8 g KCl […]
Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Fixation Buffers Expand 2% paraformaldehyde in PBS NaCl […]
"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."
Jerrold R. Turner, M.D., Ph.D.
"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."
Univ.-Prof. Jay Gopalakrishnan PhD
"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."
Julie Craft Van De Weghe, PhD
"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"
Divyesh Joshi, PhD
"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."
Harshita B Gupta, PhD.
"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.
They worked very well! Thank you!"
Victoria Gorbacheva, PhD.
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